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Abstract P4 ATPases (i.e., lipid flippases) are eukaryotic enzymes that transport lipids across membrane bilayers. In plants, P4 ATPases are named Aminophospholipid ATPases (ALAs) and are organized into five phylogenetic clusters. Here we generated an Arabidopsis mutant lacking all five cluster‐2 ALAs (ala8/9/10/11/12), which is the most highly expressedALAsubgroup in vegetative tissues. Plants harboring the quintuple knockout (KO) show rosettes that are 2.2‐fold smaller and display chlorotic lesions. A similar but less severe phenotype was observed in anala10/11double KO. The growth and lesion phenotypes ofala8/9/10/11/12mutants were reversed by expressing aNahGtransgene, which encodes an enzyme that degrades salicylic acid (SA). A role for SA in promoting the lesion phenotype was further supported by quantitative PCR assays showing increased mRNA abundance for an SA‐biosynthesis geneISOCHORISMATE SYNTHASE 1(ICS1) and two SA‐responsive genesPATHOGENESIS‐RELATED GENE 1(PR1) andPR2.Lesion phenotypes were also reversed by growing plants in liquid media containing either low calcium (~0.1 mM) or high nitrogen concentrations (~24 mM), which are conditions known to suppress SA‐dependent autoimmunity. Yeast‐based fluorescent lipid uptake assays revealed that ALA10 and ALA11 display overlapping substrate specificities, including the transport of LysoPC signaling lipids. Together, these results establish that the biochemical functions of ALA8–12 are at least partially overlapping, and that deficiencies in cluster‐2 ALAs result in an SA‐dependent autoimmunity phenotype that has not been observed for flippase mutants with deficiencies in otherALAclusters. 

Abstract The lipid bilayer of biological membranes has a complex composition, including high chemical heterogeneity, the presence of nanodomains of specific lipids, and asymmetry with respect to lipid composition between the two membrane leaflets. In membrane trafficking, membrane vesicles constantly bud off from one membrane compartment and fuse with another, and both budding and fusion events have been proposed to require membrane lipid asymmetry. One mechanism for generating asymmetry in lipid bilayers involves the action of the P4 ATPase family of lipid flippases; these are biological pumps that use ATP as an energy source to flip lipids from one leaflet to the other. The model plant Arabidopsis (Arabidopsis thaliana) contains 12 P4 ATPases (AMINOPHOSPHOLIPID ATPASE1–12; ALA1–12), many of which are functionally redundant. Studies of P4 ATPase mutants have confirmed the essential physiological functions of these pumps and pleiotropic mutant phenotypes have been observed, as expected when genes required for basal cellular functions are disrupted. For instance, phenotypes associated with ala3 (dwarfism, pollen defects, sensitivity to pathogens and cold, and reduced polar cell growth) can be related to membrane trafficking problems. P5 ATPases are evolutionarily related to P4 ATPases, and may be the counterpart of P4 ATPases in the endoplasmic reticulum. The absence of P4 and P5 ATPases from prokaryotes and their ubiquitous presence in eukaryotes make these biological pumps a defining feature of eukaryotic cells. Here, we review recent advances in the field of plant P4 and P5 ATPases. 

Long-duration gamma-ray bursts (GRBs) are powerful cosmic explosions, signaling the death of massive stars. Among them, GRB 221009A is by far the brightest burst ever observed. Because of its enormous energy (E_iso≈ 10⁵⁵erg) and proximity (z≈ 0.15), GRB 221009A is an exceptionally rare event that pushes the limits of our theories. We present multiwavelength observations covering the first 3 months of its afterglow evolution. The x-ray brightness decays as a power law with slope ≈t^−1.66, which is not consistent with standard predictions for jetted emission. We attribute this behavior to a shallow energy profile of the relativistic jet. A similar trend is observed in other energetic GRBs, suggesting that the most extreme explosions may be powered by structured jets launched by a common central engine. 

We present a map of the total intrinsic reddening across ≃ 90 deg2 of the Large Magellanic Cloud (LMC) derived using optical (ugriz) and near-infrared (IR; YJKs) spectral energy distributions (SEDs) of background galaxies. The reddening map is created from a sample of 222,752 early-type galaxies based on the lephare χ2 minimization SED-fitting routine. We find excellent agreement between the regions of enhanced intrinsic reddening across the central (4 × 4 deg2) region of the LMC and the morphology of the low-level pervasive dust emission as traced by far-IR emission. In addition, we are able to distinguish smaller, isolated enhancements that are coincident with known star-forming regions and the clustering of young stars observed in morphology maps. The level of reddening associated with the molecular ridge south of 30 Doradus is, however, smaller than in the literature reddening maps. The reduced number of galaxies detected in this region, due to high extinction and crowding, may bias our results towards lower reddening values. Our map is consistent with maps derived from red clump stars and from the analysis of the star formation history across the LMC. This study represents one of the first large-scale categorisations of extragalactic sources behind the LMC and as such we provide the lephare outputs for our full sample of ∼ 2.5 million sources. 

Abstract The Gravitational-Wave Transient Catalog (GWTC) is a collection of short-duration (transient) gravitational-wave signals identified by the LIGO–Virgo–KAGRA Collaboration in gravitational-wave data produced by the eponymous detectors. The catalog provides information about the identified candidates, such as the arrival time and amplitude of the signal and properties of the signal’s source as inferred from the observational data. GWTC is the data release of this dataset, and version 4.0 extends the catalog to include observations made during the first part of the fourth LIGO–Virgo–KAGRA observing run up until 2024 January 31. This Letter marks an introduction to a collection of articles related to this version of the catalog, GWTC-4.0. The collection of articles accompanying the catalog provides documentation of the methods used to analyze the data, summaries of the catalog of events, observational measurements drawn from the population, and detailed discussions of selected candidates. 

Abstract We report the observation of gravitational waves from two binary black hole coalescences during the fourth observing run of the LIGO–Virgo–KAGRA detector network, GW241011 and GW241110. The sources of these two signals are characterized by rapid and precisely measured primary spins, nonnegligible spin–orbit misalignment, and unequal mass ratios between their constituent black holes. These properties are characteristic of binaries in which the more massive object was itself formed from a previous binary black hole merger and suggest that the sources of GW241011 and GW241110 may have formed in dense stellar environments in which repeated mergers can take place. As the third-loudest gravitational-wave event published to date, with a median network signal-to-noise ratio of 36.0, GW241011 furthermore yields stringent constraints on the Kerr nature of black holes, the multipolar structure of gravitational-wave generation, and the existence of ultralight bosons within the mass range 10⁻¹³–10⁻¹²eV. 

Abstract On 2023 November 23, the two LIGO observatories both detected GW231123, a gravitational-wave signal consistent with the merger of two black holes with masses $137_{-18}^{+23}{M}_{\odot }$and $101_{-50}^{+22}{M}_{\odot }$(90% credible intervals), at a luminosity distance of 0.7–4.1 Gpc, a redshift of $0.40_{-0.25}^{+0.27}$, and with a network signal-to-noise ratio of ∼20.7. Both black holes exhibit high spins— $0.90_{-0.19}^{+0.10}$and $0.80_{-0.52}^{+0.20}$, respectively. A massive black hole remnant is supported by an independent ringdown analysis. Some properties of GW231123 are subject to large systematic uncertainties, as indicated by differences in the inferred parameters between signal models. The primary black hole lies within or above the theorized mass gap where black holes between 60–130M_⊙should be rare, due to pair-instability mechanisms, while the secondary spans the gap. The observation of GW231123 therefore suggests the formation of black holes from channels beyond standard stellar collapse and that intermediate-mass black holes of mass ∼200M_⊙form through gravitational-wave-driven mergers. 

ABSTRACT We present a map of the total intrinsic reddening across ≃34 deg2 of the Small Magellanic Cloud (SMC) derived using optical (ugriz) and near-infrared (IR; YJKs) spectral energy distributions (SEDs) of background galaxies. The reddening map is created using a subsample of 29 274 galaxies with low levels of intrinsic reddening based on the lephare χ2 minimization SED-fitting routine. We find statistically significant enhanced levels of reddening associated with the main body of the SMC compared with regions in the outskirts [ΔE(B − V) ≃ 0.3 mag]. A comparison with literature reddening maps of the SMC shows that, after correcting for differences in the volume of the SMC sampled, there is good agreement between our results and maps created using young stars. In contrast, we find significant discrepancies between our results and maps created using old stars or based on longer wavelength far-IR dust emission that could stem from biased samples in the former and uncertainties in the far-IR emissivity and the optical properties of the dust grains in the latter. This study represents one of the first large-scale categorizations of extragalactic sources behind the SMC and as such we provide the lephare outputs for our full sample of ∼500 000 sources. 

ABSTRACT The periphery of the Small Magellanic Cloud (SMC) can unlock important information regarding galaxy formation and evolution in interacting systems. Here, we present a detailed study of the extended stellar structure of the SMC using deep colour–magnitude diagrams, obtained as part of the Survey of the MAgellanic Stellar History (SMASH). Special care was taken in the decontamination of our data from Milky Way (MW) foreground stars, including from foreground globular clusters NGC 362 and 47 Tuc. We derived the SMC surface brightness using a ‘conservative’ approach from which we calculated the general parameters of the SMC, finding a staggered surface brightness profile. We also traced the fainter outskirts by constructing a stellar density profile. This approach, based on stellar counts of the oldest main-sequence turn-off stars, uncovered a tidally disrupted stellar feature that reaches as far out as 12 deg from the SMC centre. We also serendipitously found a faint feature of unknown origin located at ∼14 deg from the centre of the SMC and that we tentatively associated with a more distant structure. We compared our results to in-house simulations of a 1 × 109 M⊙ SMC, finding that its elliptical shape can be explained by its tidal disruption under the combined presence of the MW and the Large Magellanic Cloud. Finally, we found that the older stellar populations show a smooth profile while the younger component presents a jump in the density followed by a flat profile, confirming the heavily disturbed nature of the SMC. 

Abstract The SDSS-IV Apache Point Observatory Galactic Evolution Experiment (APOGEE) survey has obtained high-resolution spectra for thousands of red giant stars distributed among the massive satellite galaxies of the Milky Way (MW): the Large and Small Magellanic Clouds (LMC/SMC), the Sagittarius Dwarf Galaxy (Sgr), Fornax (Fnx), and the now fully disrupted Gaia Sausage/Enceladus (GSE) system. We present and analyze the APOGEE chemical abundance patterns of each galaxy to draw robust conclusions about their star formation histories, by quantifying the relative abundance trends of multiple elements (C, N, O, Mg, Al, Si, Ca, Fe, Ni, and Ce), as well as by fitting chemical evolution models to the [ α /Fe]–[Fe/H] abundance plane for each galaxy. Results show that the chemical signatures of the starburst in the Magellanic Clouds (MCs) observed by Nidever et al. in the α -element abundances extend to C+N, Al, and Ni, with the major burst in the SMC occurring some 3–4 Gyr before the burst in the LMC. We find that Sgr and Fnx also exhibit chemical abundance patterns suggestive of secondary star formation epochs, but these events were weaker and earlier (∼5–7 Gyr ago) than those observed in the MCs. There is no chemical evidence of a second starburst in GSE, but this galaxy shows the strongest initial star formation as compared to the other four galaxies. All dwarf galaxies had greater relative contributions of AGB stars to their enrichment than the MW. Comparing and contrasting these chemical patterns highlight the importance of galaxy environment on its chemical evolution.